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p-Creode

p-Creode takes continuous single-cell data to generate transition trajectories and then use resampling to quantitatively score the quality and stability of computed results (Herring et al., Cell Systems, 2018)

dropkick

Self contained machine learning algorithm for identifying true cells from cell barcodes in high ambient background samples (Heiser et al., Genome Research, 2021)

MIRIAM

Multiplex microscopy imaging segmentation algorithm focusing on membrane connectivity and designed for columnar epithelial cells (McKinley et al., Cytometry A, 2022)

sc-UniFrac

sc-UniFrac compares single-cell landscape diversity between samples by representing the global and local structure of single-cell data as trees and then performing hierarchical tree comparisons (Liu et al., PLoS Biology, 2018)

DR-Structure

Quantitative comparisons of multi-dimensional data structures when transformed by different dimension reduction techniques (Heiser and Lau, Cell Reports, 2020)

pyNVR

pyNVR is a faster way to select relevant features as input for p-Creode and other trajectory analysis algorithms, selecting gene (features) based on small local variances (Chen et al., Bioinformatics, 2019)

Open Source scRNA-seq Processing

Open source pipeline to process scRNA-seq data from FastQ files to clustering/dimension reduction – containerized (Chen et al., Star Protocols, 2021)

p-Creode score for comparing graphs of different sizes

An implementation of the p-Creode score that compares graphs with different nodes and edges, but across different samples that may have unbalanced number of nodes (Liu et al., PLoS Biology, 2018)

Cell manuscript data processing

Code to reproduce analyses in Chen et al., Cell, 2021

Semi-supervised heuristic cell filtering

Our pipeline to semi-automatically to filter cells from empty barcodes for downstream analyses – deprecated version – new version wrapped in “Open source scRNA-seq processing” (Chen et al., Star Protocols, 2021)

Single-cell isolation from tissue with cold protease

Single-cell isolation from colonic mucosa